Arjun Rampal and pregnant girlfriend Gabriella Demetriades enjoy summer vibes. See pic

first_imgArjun Rampal is all heart for Gabriella Demetriades. The actor, who recently announced girlfriend Gabriella’s pregnancy, posted an adorable picture with her on Instagram.The actor captioned the photo, “Summer vibes.”Earlier, Arjun took to Instagram to reveal Gabriella’s preganacy with a loved-up post. He shared a picture with her, where she is seen flaunting her baby bump, and said, “Blessed to have you and start all over again….thank you baby for this baby.”Before dating Gabriella, Arjun Rampal was married to Mehr Jesia. The couple, however, announced their separation on July 25, 2018. The couple announced their decision to end the marriage in a joint statement.Arjun has two daughters with Mehr – Mahikaa, who is 17-year old, and Myra, who is 13.Initially, Arjun kept his relationship with Gabriella under the wraps. But the Rock On actor made it official through an Instagram post.Rumour has it that Arjun met Gabriella during an Indian Premiere League afterparty in 2009, but they reconnected and began dating only a few years ago. She was by his side when his mother, Gwen Rampal, passed away last year.It was earlier reported that Mehr is taking Gabriella’s preganancy news sportingly. According to a report in Mumbai Mirror, though Arjun and Mehr haven’t filed for divorce yet, Mehr’s friends say that she has accepted the fact that Arjun has moved on.advertisement”They are still working out the financial terms of the separation,” Mehr’s friend told the daily. “Mehr is a wonderful mother and wants to do what works best for her children,” her friend added.Also Read | Arjun Rampal’s ex-wife Mehr Jesia is fine with his girlfriend Gabriella Demetriades’s pregnancy: ReportAlso See | Arjun Rampal announces girlfriend Gabriella Demetriades is pregnant: Thank you baby for this babyALSO READ | Has Arjun Rampal moved in with his girlfriend Gabriella Demetriades?ALSO READ | Inside story of Arjun Rampal and Mehr Jesia’s separationALSO WATCH | Ranbir-Katrina to Farhan-Adhuna, big Bollywood break-ups of 2016last_img read more

Calgary quadruple homicide Owner of burnedout car believed to be main target

first_imgCALGARY – A man and two sisters found dead in a burned-out car may not have been the intended targets in what Calgary police are describing as a brutal and ruthless quadruple homicide.Investigators are exploring the possibility that Cody Pfeiffer, 25, Glynnis Fox, 36, and Tiffany Ear, 39, were “simply at the wrong place and at the wrong time with the wrong people,” acting Insp. Paul Wozney said Thursday.Lorenzo Ear, the younger brother of the two women, said his sisters leave behind 16 children between them.“The younger ones, they don’t know yet,” he said. “That’s going to be something that we as family are going to have to find out how to explain to them, that their mothers are no longer around.”The bodies of Pfeiffer, Fox and Ear were found Monday after firefighters extinguished a burning 2011 Chevrolet Cruze at a construction site in a new subdivision on Calgary’s northwestern edge.Wozney, with the major crimes unit, said it’s believed the Cruze’s owner, Hanock Afowerk, 26, was the target.Police confirmed that Afowerk was found dead in a rural area west of Calgary on Wednesday and that it was a homicide. They had earlier appealed to the public for help finding him and expressed concern for his safety.It’s possible Fox and Ear — from the Stoney Nakoda Nation west of Calgary — were caught up in a targeted attack against Afowerk, police said.All four victims suffered significant traumatic injuries, but Wozney declined to elaborate.“I will say that it … certainly has been surprising to some very seasoned investigators.”Police believe multiple people may have been involved and that it’s possible there are several different crime scenes.“We know that there’s people in the community that have information regarding this event. We know that people know what happened. If they’re scared, if they are in any way hesitant to contact us, they can do so anonymously through Crime Stoppers or through our tip line,” said Wozney.It appears the sisters got to know Pfeiffer and Afowerk recently, he added.Lorenzo Ear said his sisters were generous, caring and kind mothers who loved their children. Tiffany had nine, and had recently become a grandmother.“I believe she really enjoyed that role. Even though it made her seem a little bit older, she was still happy.”Glynnis had seven children and was about to become a grandmother when she died.Both women were working on upgrading their education, their brother said, adding Tiffany loved books and Glynnis had a passion for learning about First Nations culture.They wanted to one day find jobs as teachers or in other professions that would allow them to help people, he said.An aunt who did not want her name published said in an email that Tiffany had been through a lot and struggled to make ends meet at times.“She kept her head up, always positive and recently completed a treatment program in December of 2016 and changed her life around,” said the aunt.The sisters were among 11 siblings, Lorenzo said, adding he wants to speak out about them to help the families of missing and murdered Indigenous women and girls.The family has been gathering at Tiffany’s home to get it ready for the wake, finding comfort in sprucing up the lawn and putting a fresh coat of paint on the inside.“What we’re doing is being a family unit, being together, being closer, laughing with each other, helping each other.”last_img read more

Tropical Depression Five Creeping Up On Us

first_img Weekend Weather Watch – storms and general TCI weather for weekend Facebook Twitter Google+LinkedInPinterestWhatsApp System becomes Tropical Storm Danny Tropical Watch with three systems being monitored Related Items:atlantic ocean, tropical depression Facebook Twitter Google+LinkedInPinterestWhatsAppTROPICAL WEATHER OUTLOOKNWS NATIONAL HURRICANE CENTER MIAMI FL200 AM EDT FRI SEP 5 2014For the North Atlantic…Caribbean Sea and the Gulf of Mexico:1. An area of low pressure, associated with a tropical wave, locatedjust south of the Cape Verde Islands is producing disorganizedshowers and thunderstorms. Some slow development of this system ispossible over the next several days while it moves westward at about15 mph. This system could bring locally heavy rain and gusty windsin squalls to portions of the Cape Verde Islands today.* Formation chance through 48 hours…low…20 percent.* Formation chance through 5 days…medium…30 percent. Recommended for youlast_img read more

The story behind the Crazy Rich Asians Black Panther viral cast photo

first_imgThe Crazy Rich Asians and Black Panther viral joint cast photo was made possible by Harry Shum Jr. Steven Lam When the casts of Crazy Rich Asians and Black Panther posed together for a photo at the Screen Actors Guild Awards in January, the shot immediately went viral. The picture — which includes Crazy Rich Asians stars Constance Wu and Harry Shum Jr., as well as Black Panther stars Chadwick Boseman and Lupita Nyong’o — lit up the internet with delight.Turns out the moment was made possible by Shum, who just wanted to show his appreciation for the Black Panther ensemble, which won best cast in a motion picture at the awards ceremony.”I remember going to some [Crazy Rich Asians] cast members and saying, ‘Yo, let’s go say congratulations to the Black Panther cast,'” Shum said in an interview. “And then that happened.” “It was just this thing that started. People saw us mingling, and it was like, this is a perfect example of what Hollywood should be like, what it needs to do,” he continued. “It’s not just about your own community. How do you reach out with the community in mind, and collaborate or just support?”Shum spoke at a reception Thursday evening in Los Angeles for the Gold House A100, a list that honors Asians and Asian Americans in tech, entertainment and business. The list, announced earlier this month, includes several Crazy Rich Asians stars, including Shum, Wu, Henry Golding, Awkwafina and director Jon M. Chu. On Friday, Gold House also gave Chu its inaugural A1 award for the “single most impactful Asian of the year.”Crazy Rich Asians and Black Panther were two of last year’s cultural milestones. The two films represented a push in Hollywood to tell more diverse stories and get more people of color on screen and behind the camera. Both movies were critical and commercial successes, and proved that movies with predominantly nonwhite casts could do well at the box office. 2:02 0 Now playing: Watch this: 2019 movies to geek out over Share your voice Danai Gurira, woman of actioncenter_img For Shum, the joint cast photo was an encapsulation of that push for representation. “It’s just a celebration of being there at this award show when so many faces have been erased or have been invisible,” he said. “And just to be there and see [the Black Panther cast] holding those trophies, it’s special.”Outside of entertainment, other heavy hitters honored by Gold House include Google CEO Sundar Pichai, Momofuku chef David Chang and Toronto Raptors point guard Jeremy Lin.Gold House also tries to ensure the success of projects driven by Asian people. A campaign by the group called #GoldOpen aims to buoy opening weekends by urging donors to buy out movie theaters and snap up tickets in bulk. The group made a big push around Crazy Rich Asians last year, which has grossed almost $240 million at the box office to date, and is the top grossing romantic comedy in 10 years. Tags 77 Photos Post a comment Culture Black Pantherlast_img read more

GCC polls rigging was not protested for strategic reason BNP

first_imgMirza AbbasBNP senior leader Mirza Abbas on Saturday said their party did not put up any resistance against the ruling party’s various irregularities in Gazipur and Khulna polls due to a strategic reason, reports UNB.”The ruling party won the Gazipur and Khulna polls through rigging. The leaders and activists of BNP were driven out from their areas with arrest, threats and intimidation after the announcement of the election schedule,” he said.Speaking at a discussion, he further said, “BNP didn’t create any resistance due to a strategic reason. But we’ll come up with the resistance in the future.”Jatiyatabadi Help Cell arranged the programme at Dhaka Reporters’ Unity. Six families of those subjected to enforced disappearance, killing and repression by ruling party and law enforcers were provided with financial assistance at the programme.Abbas, a BNP standing committee member, said he does not disagree with his party colleagues that their party needs to go to polls. “But I think it’s more crucial to free our chairperson Khaleda Zia than joining the polls.”He also feared that the national election will be held in the same style of Khulna and Gazipur if it is held without the participation of Khaleda Zia. “No one will be able to resist BNP’s victory if we can join it freeing Khaleda Zia.”The BNP leader said they have got information that the government is making a list of BNP leaders to suppress them. “This list will turn futile in the face of BNP’s movement.”last_img read more

Sodium selective DNAzyme sensor

first_img Journal information: Proceedings of the National Academy of Sciences © 2015 Phys.org However, studying sodium in real-time in living cells has proved difficult. Most biological fluorescence sensors are not selective for sodium, often binding to potassium, or are not feasible in a cellular environment, requiring organic solvents. Other applications cannot provide real-time data. A team from the Departments of Biochemistry, Chemistry, and Materials Science and Engineering at the University of Illinois at Urbana-Champaign has devised a biological fluorescent sensor that is selective for sodium ions and has demonstrated its ability to sense sodium in living cells. Their work was recently published in the Proceedings of the National Academy of Sciences.The University of Illinois team took advantage of recent developments in developing deoxy-ribozymes, or DNAzymes. DNAzymes are a kind of catalytic DNA that is obtained in the lab using a high throughput selection process. Prior research has demonstrated how DNAzymes can be used as metal ion sensors by designing them to have fluorescent labels that are only “turned on” when the DNAzyme binds the target metal and catalyzes enzymatic reactions. While these studies have demonstrated DNAzymes that can bind monovalent ions, such as Na+ or K+, thus far they have not been selective for sodium over potassium. The University of Illinois team identified and tested a DNAzyme that is more than 1,000-fold selective for sodium ion over other metals. Furthermore, their DNAzyme can detect sodium concentrations that are within the range typically seen in cells (0.135-50mM), and their detection method is fast enough that real-time studies can be conducted.DNAzymes can be converted to a fluorescent sensor by placing a fluorophore on one portion of the DNAzyme and a fluorescence quencher on another potion. As long as the fluorophore and the quencher are in contact, only background fluorescence is observed. Once the DNAzyme binds the target metal ion, Na+ in this case, it initiates cleaving a loop of DNA at a particular nucleotide, releasing the substrate portion with the fluorophore. It is separated from the quencher resulting in a fluorescent signal. New test for germs: Fluorescing DNAzymes detect metabolic products from bacteria Citation: Sodium selective DNAzyme sensor (2015, May 11) retrieved 18 August 2019 from https://phys.org/news/2015-05-sodium-dnazyme-sensor.html Scheme of the decaging process for the photolabile Na+-specific DNAzyme. Credit: (c) 2015 PNAS, doi: 10.1073/pnas.1420361112 Explore further More information: “In vitro selection of a sodium-specific DNAzyme and its application in intracellular sensing” PNAS, DOI: 10.1073/pnas.1420361112AbstractOver the past two decades, enormous progress has been made in designing fluorescent sensors or probes for divalent metal ions. In contrast, the development of fluorescent sensors for monovalent metal ions, such as sodium (Na+), has remained underdeveloped, even though Na+ is one the most abundant metal ions in biological systems and plays a critical role in many biological processes. Here, we report the in vitro selection of the first (to our knowledge) Na+-specific, RNA-cleaving deoxyribozyme (DNAzyme) with a fast catalytic rate [observed rate constant (kobs) ∼0.1 min−1], and the transformation of this DNAzyme into a fluorescent sensor for Na+ by labeling the enzyme strand with a quencher at the 3′ end, and the DNA substrate strand with a fluorophore and a quencher at the 5′ and 3′ ends, respectively. The presence of Na+ catalyzed cleavage of the substrate strand at an internal ribonucleotide adenosine (rA) site, resulting in release of the fluorophore from its quenchers and thus a significant increase in fluorescence signal. The sensor displays a remarkable selectivity (>10,000-fold) for Na+ over competing metal ions and has a detection limit of 135 µM (3.1 ppm). Furthermore, we demonstrate that this DNAzyme-based sensor can readily enter cells with the aid of α-helical cationic polypeptides. Finally, by protecting the cleavage site of the Na+-specific DNAzyme with a photolabile o-nitrobenzyl group, we achieved controlled activation of the sensor after DNAzyme delivery into cells. Together, these results demonstrate that such a DNAzyme-based sensor provides a promising platform for detection and quantification of Na+ in living cells. (Phys.org)—Sodium ions are key regulators in cellular processes. The fluids in cells, whether it is water, blood plasma, or nutrients, are regulated by the sodium concentration in cells. If scientists could study sodium ions within a live cell, they would gain important insights into cellular processes including ways to reprogram these processes for biotechnological applications. DNAzymes are determined by subjecting a library of synthetic DNA candidates to in vitro binding studies using column-based and gel-based selection methods. Potential candidates are then amplified and tested until an optimal candidate is determined. Through this selection and amplification process, this group found a DNAzyme, labeled NaA43, that was selective for Na+. The next step was to make the fluorescent label. Every DNAzyme has two segments, the substrate and the enzyme strand. For this experiment, as NaA43S and NaA43E are the substrate and enzyme, respectively. The 5′ end of NaA43S was labeled with a known fluorophore, and a quencher was placed at its 3’end. An additional quencher was added to the 3′ end to ensure a minimal amount of background fluorescence. When Na+ was added, NaA43S was cleaved at the target nucleotide, and the fluorophore was released from quenchers. The result was an increase in fluorescent signal. Furthermore, fluorescence did not significantly change when twenty-two other metal ions were tested.Finally, the DNAzyme needed to be prepared for cellular insertion and detection. The process of delivering the DNAzyme into the cell could result in premature cleavage, so this team employed “photocaging” to control when the substrate was cleaved. Photocages are photoactive molecules that are placed at the cleavage site to prevent DNA substrate cleavage. When light at a certain wavelength is irradiated at the site, the photo-caged group is released, and then the substrate can be cleaved.Finally, in order to transport the DNAzyme through the cell membrane and into the cytosol, they used a class of alpha-helical cationic polypeptide that is known to facilitate transportation through the cell membrane. After four-hour incubation into living HeLa cells, NaA43ES was found to be located predominately in the cytosol and did not accumulate in other organelles. The cleavage site was “uncaged” by irradiating the cell with light (365 nm) for thirty minutes. Then they enhanced the sodium levels in the cells. As sodium ions traveled from the extracellular matrix to within the cell, fluorescence measurements increased during this time, demonstrating intracellular Na+ detection in living cells.This work reports the first use of a DNAzyme to make a real-time, selective sodium ion sensor that can be used in living cells. Since sodium selectivity has been difficult to achieve, and these studies will not only allow for additional studies on cellular activity but may also shed light on ion selectivity, in general. This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.last_img read more

CLIAs ChooseCruise campaign replaces Plan a Cruise Month

first_imgCLIA’s #ChooseCruise campaign replaces Plan a Cruise Month WASHINGTON, D.C. — CLIA is urging travellers to #ChooseCruise with a new global marketing initiative kicking off this October that includes a new tool kit for travel agents.Formerly known as Plan a Cruise Month, #ChooseCruise “unites the global cruise industry – including cruise lines, travel agents and global CLIA offices – through an integrated campaign to promote positive messaging about why to choose and book a cruise vacation,” says CLIA.“The #ChooseCruise campaign is a way to highlight the many amazing reasons why travellers choose a cruise for vacation,” adds Cindy D’Aoust, President and CEO, CLIA. “From spa experiences onboard to one-of-a-kind adventures on land, cruise travel offers the chance to check off bucket list items in a fun, carefree way.”Throughout October CLIA will leverage daily observances – everything from National Taco Day and Sweetest Day to Picasso’s Birthday and Great Books Day – as well as holidays to highlight the many reasons to #ChooseCruise and integrate cruising into popular social conversations throughout the month. CLIA’s social channels have more information and sharable content:INSTAGRAM: https://www.instagram.com/cliaglobal/FACEBOOK: https://www.facebook.com/CLIAGlobal/ TWITTER: https://twitter.com/CLIAGlobal LINKEDIN: https://www.linkedin.com/company/clia-cruise-lines-international-association-/Cruise lines will be offering exclusive deals and promotions and agents can access these deals in the #ChooseCruise tool kit starting Sept. 11.CLIA will also host a #ChooseCruise webinar for travel agents on Sept. 11, presented by Charles Sylvia, Vice President, Membership and Trade Relations. Other #ChooseCruise travel agent-centric events include weekly Twitter events Sept. 26 – Oct. 24, where agents can learn the latest about the initiative and engage with cruise lines with a chance to win various prizes. CLIA will also provide a Travel Agent Tool Kit that includes marketing materials, press release templates, social media support and logos, available starting Sept. 11. Share Thursday, August 30, 2018 Travelweek Group center_img Posted by Tags: CLIA << Previous PostNext Post >>last_img read more

Register now for ACVs Europe Regional training sessions

first_img UPDATE: Air Canada Vacations has made a correction on the Winnipeg date training and added a number of additional training dates. The correction and additional dates have been added below.MONTREAL — Travel agents across the country are invited to attend Air Canada Vacations’ Europe Regional training sessions ahead of its 2019 Europe Collection launch.At each session, ACV’s sales team will share highlights of the product lineup along with its new programs and partnerships. Portugal Tourism, the main event sponsor, will present alongside other Europe suppliers like Avis Rent A Car, Azamara Cruises, Insight Vacations and Royal Caribbean International.“Our Europe collection continues to expand and the resulting support from our trade partners has been fantastic,” said Dana Gain, Senior Director of Sales, Groups & Partnerships at ACV. “We’re already seeing a terrific response from the trade and a lot of great feedback. Make sure you block your seat while there’s still space!”Agents can register via the following links:January 15, Formation Rive-Sud: https://aircanadavacations.formstack.com/forms/europe_regional_training_south_shoreJanuary 15, Kelowna: westevents@vacv.com << Previous PostNext Post >> Share Travelweek Group January 15, Hamilton: https://aircanadavacations.formstack.com/forms/europe_regional_training__hamiltonJanuary 15, Oshawa: https://aircanadavacations.formstack.com/forms/air_canada_vacations__oshawa__europe_reginoal_training_15_january_2019_breakfast_eventJanuary 16, Formation Granby: https://aircanadavacations.formstack.com/forms/europe_regional_training_granbyJanuary 16, Victoria: westevents@vacv.comJanuary 17, Langley: westevents@vacv.comJanuary 17, Barrie: https://aircanadavacations.formstack.com/forms/europe_regional_training_barrieJanuary 17, St. Catherine’s: https://aircanadavacations.formstack.com/forms/europe_regional_training__st_catherinesJanuary 17, Formation Saguenay: https://aircanadavacations.formstack.com/forms/europe_regional_training_saguenayJanuary 23, Formation QC avec AC: https://aircanadavacations.formstack.com/forms/europe_regional_training_quebecJanuary 25, Gatineau: https://aircanadavacations.formstack.com/forms/europe_regional_training_gatineauJanuary 31, Halifax: https://aircanadavacations.formstack.com/forms/europe_regional_training_halifaxFebruary 6, St. John’s: https://aircanadavacations.formstack.com/forms/europe_regional_training_st_johnsFebruary 27, Winnipeg: https://aircanadavacations.formstack.com/forms/europe_regional_training_winnipegMore news:  FIVE FESTIVE FOODS TO TRY AT EUROPE’S CHRISTMAS MARKETSAir Canada Vacations is reminding travel agents that when clients book 180 days prior to departure, they save up to $1,000 per couple. When they book 120 days prior, they save up to $500 per couple, and $300 per couple when booking 90 days prior. This offer is valid on bookings made between Nov. 5, 2018 and Dec. 31, 2019, for travel from Jan. 1-Dec. 31, 2019. Register now for ACV’s Europe Regional training sessions Tags: ACV, Air Canada Vacations, Europe, Training Posted by Monday, January 14, 2019 last_img read more